IL17F antibody

Catalog No. ABIN465701

The Rabbit Polyclonal anti-IL17F antibody has been validated for WB, ELISA and Neut. It is suitable to detect IL17F in samples from Human.

Quick Overview for IL17F antibody (ABIN465701)

Target: IL17F (Interleukin 17F (IL17F))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL17F antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL17F Antibody

Specificity: Human IL-17F

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IL-17F.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (0.9 - 1.2 μg/mL), WB

Usage: Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-17F (50 ng/mL), a concentration of 0.9-1.2 μg/mL of this antibody is required. ELISA: To detect hIL-17F by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-17F (LS-C104642) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-17F. Western Blot: To detect hIL-17F by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17F is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with sterile water.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2. Reconstitute with sterile water.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL17F

Target: IL17F (Interleukin 17F (IL17F))

Alternative Name: IL17F

Background: Name/Gene ID: IL17F

Synonyms: IL17F, CANDF6, Cytokine ML-1, IL-17F, Interleukin-17F, Interleukin 17F, Interleukin-24, ML-1, ML1, Mutant IL-17F

Gene ID: 112744

UniProt: Q96PD4

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity